This study was designed tocompare the potential differences in the colonization of Chironomidae pupalfauna in various leaf packs. The study was carried out in Tunca River (Edirne).Three stations were selected in the river and 5 different leaf packs (Platanus orientalis L., Ulmus leavis Pall., Morus alba L., Juglansregia L., and artificial Buxussp.) were used to take samples. 20 kg potato bags were used during leavespackaging, prepared 5 packages of each leaves and a total of 25 packets wereplaced in each of the stations. Chironomidae pupal samples were collected fromJune 2012 to October 2012. The collected samples were placed in 70% ethylalcohol, brought to the laboratory and diagnosed under stereo microscope. Then ANOVA test was used toanalyse Chironomidae pupae in dates, stations, and leaf packs and 0.05 αstatistical significance was used in all tests. When there was a meaningfuldifference, the reason was revealed by the Tukey test. Chironomidae pupae werefound at most 3, 1 and 2. stations respectively and in leaf species of artificial Buxus sp. and Ulmus leavis. Eight species wereidentified in leaf packs. The Polypedilum(Polypedilum) nubeculosum (Meigen, 1804) was found tobe the most common species.